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Stem Cell
Therapy
Stem Cell Therapy Procedure
The
actual procedure consists of inserting a fistula needle in a
peripheral line. Should vene-puncture in the peripheral line not be
possible, a central venous pressure (CVP) line in the format of an
inner jugular line, a sub-clavian line or a femoral line, depending
on the patient’s age, size condition and indication, is inserted
into the patient using local anesthesia and a return catheter into
the opposite arm." This is done by our
qualified ICU doctor / anesthetists. The CVP line is then hooked
into an apheresis machine (GAMBRO COBE Spectra) which separates a
volume of the patient's blood into 3 parts: Red Blood Cells, White
Blood Cells and Platelets. The Red Blood Cells and Platelets are
returned to the patient immediately via the machine. A small volume
of the White Blood Cells depending on the patient's age, weight,
height, size and condition are taken and converted into pluripotent
stem cells via a process of Retrodifferentiation (addition of a
monoclonal antibody to a particular cell surface contact receptor).
These cells are then given a direction to re-differentiate into the
required cell type (progenitors). After the require time of
incubation, the stem cells are then washed in a GAMBRO COBE 2991
Cell Processor and re-infused into the patient. The whole process
takes between 6 and 8 hours. The CVP line is removed an hour later
and the patient is then monitored for a period of 24 to 48 hours
and then discharged.
The whole process is non-invasive (except for the CVP line
insertion) and painless for the patient. As these are patient cells
derived from the patient (i.e. patients are their own donors), there
is no conditioning regimen, growth factors or immuno-suppression of
any kind used in this process at any time before, during or after
the procedure. This avoids any of the several complication
associated with taking immuno-suppression / growth factors and/or
conditioning regimens. |
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